Table 3.
Intracellular neutralization of virus by anti-M IgA antibodya
| Cell and antibody (20 μg in 120 μl) | Vero-IgR |
Vero |
||
|---|---|---|---|---|
| Virus titer (PFU/well) | % Reduction of virusb | Virus titer (PFU/well) | % Reduction of virusb | |
| 5H7-IgA (anti-M) | 1,846.67 ± 153.22c | 78.02 | 9,208.67 ± 710.08 | 0.00 |
| 5H7-IgG (anti-M) | 8,470.00 ± 252.39 | 0.00 | 9,378.33 ± 309.49 | 0.00 |
| Irrelevant anti-HIVgp41 IgA | 7,840.00 ± 403.64d | 6.67 | 8,608.33 ± 457.88d | 3.62 |
| No MAb | 8,400.00 ± 378.58 | 0.00 | 8,930.33 ± 307.74 | 0.00 |
a
Experiments were repeated on at least three occasions with similar results. The results of a representative experiment are shown. The data are means ± SEM (n = 3).
b
Compared to no-antibody control.
c
Mean virus titers were significantly lower (P ≤ 0.0001) than those from cells exposed to no antibody.
d
Mean virus titers were not significantly lower (P ≥ 0.05) than those from cells exposed to no antibody.