Fig. 10.

Wp-dependent protein expression is repressed in superinfected Sal BL cells. Immunoblot detection of the EBV protein EBNALP (A) or BHRF1 (B), believed to arise predominately if not exclusively from transcription originating from Wp in Sal and other Wp-restricted BL lines, is shown. Note that the antibody used to detect EBNA-LP does not detect EBNA-LP encoded by the Akata strain of EBV (as in Ak-LCL and rEBV used to superinfect Sal cells). Lysates from superinfected Sal cells were harvested ∼12 months postsuperinfection. Detection of β-actin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as loading controls.