Fig. 5.

The BHLF1 locus is not required to establish restricted latency. Comparable to superinfection with wt rEBV (Fig. 1), EBNA and LMP1 expression indicative of latency III could be detected at 1 month postsuperinfection with ΔB-S rEBV (data not shown) but as demonstrated here was no longer evident at later times (>4 months); the blot shown is of protein samples harvested at 8 months postsuperinfection. The authentic EBNA3B band is marked with an asterisk in the Kem III lane; others are common background bands, since they were also detected in the EBV-negative Mutu⁻ cells.