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. 2011 Nov;85(21):11022–11037. doi: 10.1128/JVI.00719-11

Fig. 7.

Fig. 7.

Core protein is necessary for HCV-induced YB-1 relocalization to lipid droplets. (A) Huh7.5 and subgenomic replicon-containing 9-13 cells were cultured in the presence of DMSO or 2 μM BILN2061 for 5 days. Cell extracts were prepared and analyzed by Western blotting using anti-NS3, anti-YB-1, anti-MAVS, and anti-GAPDH antibodies. GAPDH was used as a loading control. (B) 9-13 cells were grown on coverslips as described for panel A. After 5 days of culture, cells were fixed and probed with rabbit anti-YB-1 (green) and mouse anti-NS3 (red) antibodies. (C) Huh7.5 cells were transfected with plasmids encoding either wild-type JFH-1 or the del153-167 JFH-1 mutant. Following 4 days of culture, cells were fixed and probed with rabbit anti-YB-1 (green) and mouse anti-NS3 (red) antibodies. Nuclei and lipid droplets were stained with Hoechst dye (white) and LipidTOX (blue), respectively. Unmerged and merged higher-magnification images of the red selected area are shown. Yellow arrows indicate YB-1/NS3 colocalization foci. (D) Huh7.5 cells were treated as described for panel C in the presence of 1 μM BILN2061 in the culture media.