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. 2011 Nov;85(21):10989–10998. doi: 10.1128/JVI.05420-11

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Fig. 2. — Characterization of the recombinant DEVs with the H5N1 HA gene insertion. (A) DNA patterns of the DEV genome and recombinant DEVs. Purified genomic DNA was digested with the indicated enzymes and then separated by using pulsed-field gel electrophoresis. (B) Southern blotting of recombinant viruses. (C) Detection of HA protein expression in recombinant virus-infected CEFs by using immunofluorescence. (D) Western blotting of recombinant DEV expressing the HA protein of A/duck/Anhui/1/06 (H5N1) virus. (E) Detection of the HA gene insertion in the recombinant viruses. The numbers show the passages of the recombinant viruses. The marker used was DL15000. (F) Detection of HA protein expression in recombinant virus-infected CEFs by using immunofluorescence. The recombinant viruses used were passaged in CEFs for 20 passages.