Fig. 5.

Upregulation of EZH2 and loss of H3K27me3 in HSILs. Immunofluorescence costaining of EZH2 and H3K27me3 in HSILs and normal biopsy specimens. Green, H3K27me3; red, EZH2; merge, DAPI plus H3K27me3. High levels of EZH2 were shown to correlate with reduced H3K27me3 staining in HSILs, while the reverse was observed in normal biopsy specimens. While EZH2 was located predominantly in the nucleus in the basal cells of normal tissue, more abundant and stronger nuclear staining was observed throughout epithelial layers of HSILs. The figure is representative of three independent sets of matched HSILs and normal biopsy specimens.