Killing activity and stability of IMB-2 (5.65 μM) in the presence of saliva. (A) Survival of cells in saliva exposed to IMB-2 with or without addition of 1 mM EDTA compared to no saliva. (B) SDS-PAGE analysis of peptide degradation in the presence of saliva with or without addition of 1 mM EDTA. The peptide bands were quantitated by densitometry to calculate percent degradation compared to the 0-min samples. Lane 1, low-molecular-weight markers; lane 2, IMB-2 only (∼4.5 kDa) as a blank control; lanes 3 to 6, IMB-2 exposed to saliva with 1 mM EDTA for 0, 5, 10, and 15 min; lanes 7 to 10, IMB-2 exposed to saliva without EDTA for 0, 5, 10, and 15 min. The arrowhead indicates IMB-2.