Table 1.
Sequences and antimicrobial activities of targeted peptides against S. mutans
| Peptidea | Targeting domain-linker-killing domainb | MICc (μM) |
|---|---|---|
| CSP | SGSLSTFFRLFNRSFTQALGK | NA |
| NRC-4 | GWGSFFKKAAHVGKHVGKAALTHYL·NH2 | 1.0 |
| IMB-1 | TFFRLFNR-GG-GWGSFFKKAAHVGKHVGKAALTHYL·NH2 | 2.2 |
| IMB-2 | TFFRLFNR-GG-GWGSFFKKAAHVGKL·NH2 | 2.8 |
| IMB-3 | TFFRLFNR-GGG-HVGKHVGKAALTHYL·NH2 | 44 |
| IMB2-Biot | Biot-TFFRLFNR-GG-GWGSFFKKAAHVGKL·NH2 | 2.8 |
| IMB2-H/S14 | Biot-TFFRLFNR-GG-GWGHFFKKAAHVGKL·NH2 | 2.8 |
a
CSP and NRC-4 are listed as the references for their original sequences. All the peptides were chemically synthesized and amidated at their C termini, except CSP. H/S indicates a His-to-Ser substitution (boldface) in the killing domain.
b
The minimal targeting domain of S. mutans CSP is underlined. -GG- or -GGG- indicates a linker sequence. Biot- indicates biotinylated peptides at the N terminus.
c
The MICs of the peptides are based on the results from triplicate experiments against S. mutans UA159. NA, not applicable.