Fig. 1.

Prevention of Huh-7 cell infection. JFH1 (A), JFH1ΔN6 (B), and H77/JFH1 (C) hepatitis C virus cell culture (HCVcc) were preincubated with different concentrations (μg/ml) of GRFT_WT or GRFT_MUT and then transferred to Huh-7 cells. Two days later, intracellular Renilla luciferase signal was quantified and normalized to the GRFT_MUT-treated group. (D) Fifty percent inhibitory concentration (IC₅₀) and 90% inhibitory concentration (IC₉₀) values were calculated via nonlinear regression analysis using a variable-slope log dose-versus-response curve with a least-squares (ordinary) fit. All conditions were tested in duplicate, and error bars represent the standard errors of the means. The 10 μg/ml concentration of griffithsin (GRFT) corresponds to 769 nM.