Fig. 4.
GRFT interacts with the viral envelope proteins. E1E2 heterodimers were coimmunoprecipitated with different concentrations of GRFTMUT (lanes 2 and 4) or GRFTWT (lanes 3 and 5). Coimmunoprecipitation without GRFT served as a negative control (lane 1). GRFTWT and GRFTMUT were added at a final concentration of 0.1 μg/ml (lanes 2 and 3) or 1 μg/ml (lanes 4 and 5). After Western blotting, E1 and E2 proteins were visualized using specific monoclonal antibodies. The positions of molecular mass markers (in kilodaltons) (PagerRuler; Fermentas) are shown to the left of the gel as a reference.