Fig. 5.

CD81 pulldown assay. HCV pseudoparticles (HCVpp) were preincubated with different concentrations (in micrograms per milliliter) of GRFT_WT or GRFT_MUT and large extracellular loop of CD81 (CD81-LEL) fused to glutathione S-transferase. Complexes were pulled down with glutathione-Sepharose beads. After Western blotting, the viral E1 and E2 proteins were visualized using specific monoclonal antibodies. The positions of molecular mass markers (in kilodaltons) (PagerRuler; Fermentas) are shown to the left of the gel as a reference.