Table 3.
ECVs for amphotericin B and six Aspergillus spp., obtained using CLSI M38-A2 and/EUCAST broth microdilution methods at 48 h
| Species | No. of isolates tested by CLSI/EUCAST methoda | MIC (μg/ml): |
Calculated statistical ECV (μg/ml)d |
|||
|---|---|---|---|---|---|---|
| Rangeb | Modec | ≥95% | ≥97.5% | ≥99% | ||
| A. fumigatus | 3,988/833 | 0.032–16 | 0.5/0.25/0.5 | 2/0.5/2e | 2/1/2 | 4/1/4 |
| A. flavus | 793/194 | 0.032–8 | 1/1/1 | 2/2/2 | 4/2/4 | 4/2/4 |
| A. nidulans | 184/69 | 0.06–32 | 1/1/1 | 4/2/4 | 4/4/4 | 4/4/8 |
| A. niger | 673/140 | 0.03–2 | 0.5/0.25/0.5 | 2/0.5/2e | 2/0.5/2 | 4/0.5/4 |
| A. terreus | 545/266 | 0.12–32 | 2/1/2 | 4/4/4 | 4/4/4 | 8/4/8 |
| A. versicolor | 135/22 | 0.032–8 | 1/1/1 | 2/2/2 | 2/4/2 | 2/4/4 |
a
CLSI aggregated data from eight laboratories and EUCAST data from a single laboratory.
b
Combined CLSI and EUCAST data.
c
MIC most frequently obtained using CLSI/EUCAST/combined CLSI and EUCAST data.
d
Calculated ECVs comprising ≥95, ≥97.5, or ≥99% of the statistically modeled population, obtained using CLSI/EUCAST/combined CLSI and EUCAST data.
e
The ECV defined using the “eyeball” method, which included at least 95% of the observed (rather than the modeled) overall distribution, was 1 μg/ml.