Fig. 2.

Biomass (μm³/μm²) and maximum thickness (μm) analysis of biofilms formed by PAO1 and PAOMS strains treated with CIP (A and B) and not treated (C and D) obtained with the COMSTAT program for the quantification of three-dimensional biofilm structures. Three time points—t0 (2-day-old biofilms), t2 (4-day-old biofilms, 2 days of CIP treatment), and t4 (6-day-old biofilms, 4 days of CIP treatment)—are represented. The results represent the means (bars) and standard deviations (error bars) of three independent experiments.