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Three-dimensional images and transversal sections of GFP (green)-tagged PAO1 and PAOMS biofilms, treated or not treated with CIP, and stained with propidium iodide (red). Images obtained at three time points—t0 (2-day-old biofilms), t2 (4-day-old biofilms, 2 days of CIP treatment), and t4 (6-day-old biofilms, 4 days of CIP treatment)—are shown for each strain.
