Fig. 5.

RC-101 is not cytotoxic to human nasal epithelia. Transwell inserts containing human nasal epithelia were incubated in the presence of RC-101 or vehicle for 24 h and assayed for cellular viability using MTT reduction (OD₅₇₀, optical density at 570 nm) (A) and trypan blue dye exclusion (B). Nasal epithelia (in 96-well plate format) were treated with increasing concentrations of RC-101 for 24 h and assayed for cellular viability using MTT reduction (C) and protease release (D) (CytoTox-GLO cytotoxicity assay). Note that RC-101 does not exhibit cytotoxicity to nasal epithelia under any of the tested conditions. n = 3 for all assays. Error bars represent the SEM.