Fig. 7.

RC-101 prevents bacterial adherence to organotypic airway epithelial tissue but does not exhibit cytotoxicity or induce a proinflammatory response. (A) Total CFU counts when nasal carriage strain D20-7 was inoculated for 9 h on organotypic human airway epithelia in the presence of RC-101. Organotypic airway epithelia were also treated with RC-101 or vehicle for 24 (B) and 72 (C) h and assayed for cellular viability using MTT reduction. (D) Following treatment of airway epithelia with RC-101 (20 μg/tissue) for 72 h, cytokine production was assessed. Shown are nine representative plots of the 27 cytokines assayed. Note that RC-101 does not significantly alter expression profiles for any of the tested cytokines. Limit of detection for data shown in panel A is 18 (i.e., log₁₀ = 1.26) total CFU. n ≥ 3. Error bars represent SEM. * indicates the starting inoculum. **, P < 0.001. P values indicate statistical significance compared to vehicle-treated samples.