Table 1.
Primers used in this study
| Primer | Sequencea | Nucleotide positionb |
|---|---|---|
| ampC-F | 5′-ATG ACG AAA GTG AAC CGC-3′ | 1-18 |
| ampC-R | 5′-CCT GGA CGA TGT GGT AAG-3′ | 1103-1120 |
| CTX-M-3F | 5′-ACC GTC ACG CTG TTG TTA G-3′ | 47-64 |
| CTX-M-3R | 5′-CTT TCT GCC TTA GGT TGA G-3′ | 806-824 |
| IMP-1F | 5′-ACC GCA GCA GAG TCT TTG CC-3′ | 49-68 |
| IMP-1R | 5′-ACA ACC AGT TTT GCC TTA CC-3′ | 616-635 |
| IMP-2F | 5′-GTT TTA TGT GTA TGC TTC C-3′ | 16-34 |
| IMP-2R | 5′-AGC CTG TTC CCA TGT AC-3′ | 677-693 |
| VIM-2F | 5′-ATG TTC AAA CTT TTG AGT AAG-3′ | 1-21 |
| VIM-2R | 5′-CTA CTC AAC GAC TGA GCG-3′ | 784-801 |
| NDM-1F | 5′-TTG CCC AAT ATT ATG CAC CC-3′ | 7-26 |
| NDM-1R | 5′-ATT GGC ATA AGT CGC AAT CC-3′ | 407-426 |
| 16S rRNA-10F | 5′-GTT TGA TCC TGG CTC A-3′ | 11-26 |
| 16S rRNA-800R | 5′-TAC CAG GGT ATC TAA TCC-3′ | 785-802 |
| SMB-F | 5′-CAG CAG CCA TTC ACC ATC TA-3′ | 79-98 |
| SMB-R | 5′-GAA GAC CAC GTC CTT GCA CT-3′ | 551-570 |
| SMB-CloF | 5′-CCC AAGCTT TCC GCC GAC TTG GCG CAG-3′ | |
| SMB-CloR | 5′-GGGGTACCA AGA CCG ATT TAG CCG GC-3′ | |
| PET-1 | 5′-GGA ATT CCATATGAA AAT CAT CGC TTC CC-3′ | |
| PET-2 | 5′-CCC AAGCTT TCA GCG TTT CTC GCT GGC C-3′ |
a
Underlines indicate the sites for restriction endonuclease.
b
Position numbers correspond to the nucleotides of the coding sequences. Position numbers are assigned to the primers that amplify the internal region of the coding sequences.