Table 3.
QTLs for seed oil traits and eQTLs for OleI, OleII and OleIII expressions in jatropha
| Trait | QTLa | Linkage | Marker | Positionb | LOD | R2 c | Additive |
|---|---|---|---|---|---|---|---|
| (eQTL) | Group | cM | Peak | (%) | Effectsd | ||
| C16:0 (%) | qC16:0-2 | 2 | Jcuint143 | 47.4 | 2.6 | 0.1 | 1.36 |
| qC16:0-7 | 7 | Jatr802 | 52.1 | 3.1 | 7.4 | 1.42 | |
| qC16:0-9 | 9 | Jatr859 | 15 | 2.6 | 7.2 | 1.39 | |
| C18:0 (%) | qC18:0-2 | 2 | curcin2 | 52.6 | 2.6 | 5.3 | -0.69 |
| qC18:0-5 | 5 | Jatr746 | 37.3 | 6.9 | 13 | 1.15 | |
| qC18:0-6 | 6 | Jcuint036 | 64 | 3.9 | 7.1 | -0.84 | |
| qC18:0-7 | 7 | Jatr883 | 40.3 | 2.3 | 4 | 0.59 | |
| qC18:0-9 | 9 | Jatr859 | 0 | 9.2 | 17.9 | 1.26 | |
| C18:1 (%) | qC18:1-1 | 1 | Jcuint057 | 0 | 18.4 | 36 | 11.69 |
| qC18:1-5 | 5 | Jatr739 | 45.1 | 2.3 | 3.4 | -3.77 | |
| qC18:1-10 | 10 | Jcuint180 | 15.2 | 4 | 5.9 | 4.75 | |
| C18:2 (%) | qC18:2-1 | 1 | Jcuint057 | 0 | 16.5 | 34.1 | -12.07 |
| qC18:2-6 | 6 | Jatr301 | 15 | 2.4 | 3.8 | 4.3 | |
| qC18:2-10 | 10 | Jcuint180 | 15.2 | 3 | 4.6 | -4.4 | |
| Total oil content (%) | qOilC-1 | 1 | Jatr722 | 55.1 | 2.3 | 4.6 | -3.72 |
| qOilC-2 | 2 | Jcuint143 | 47.4 | 2.5 | 4.9 | -3.74 | |
| qOilC-4 | 4 | Jatr872 | 29.6 | 5 | 11.1 | -5.56 | |
| qOilC-9 | 9 | Jatr698 | 18.6 | 2.5 | 5.2 | 3.74 | |
| OleI expression (ΔΔCT) | qOleI-8 | 8 | Jcuint277 | 58.2 | 1.9 | 5.3 | 1.71 |
| OleII expression (ΔΔCT) | qOleII-6 | 6 | Jatr152 | 93.4 | 2.6 | 6.4 | -2.38 |
| OleIII expression (ΔΔCT) | qOleIII-5 | 5 | Jatr739 | 46.2 | 3.1 | 11.7 | -3.06 |
a QTL (eQTL): starting with "q," followed by an abbreviation of the trait name, the name of the linkage group, and the number of QTLs (eQTLs) affecting the trait on the linkage group. OleI, OleI expression level; OleII, OleII expression level; C16:0, C18:0,18:1 and C18:2, fatty acid compositions of palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1) and Linoleic acid (C18:2); OilC: Total oil content
b Position from the first marker on each linkage group.
c Proportion of phenotypic variance (R2) explained by a QTL (eQTL).
d Estimated phenotypic effect of substituting J. integerrima alleles with J. curcas alleles at QTL (eQTL).