Figure 2.

The proteasome inhibitor MG132 confers IDO-dependent, immunosuppressive properties on IL-6 in CD8⁺ DCs. CD8⁺ DCs were conditioned by overnight incubation with IL-6. The proteasome inhibitor, MG132, was added at 10 μM for 1 h before addition of the stimuli. The IDO inhibitor, 1-MT, was added to selective cultures at 4 μM. A) Conditioned CD8⁺ DCs were pulsed with the P815AB peptide and injected into recipients hosts that were assayed for the development of P815AB-specific skin test reactivity at 2 weeks after cell transfer. Data are mean (± SD). *, P < 0.001, experimental vs control footpads. B) IDO activity was evaluated in terms of kynurenine production in culture supernatants. *, P < 0.005. In both A and B, results are mean values (± SD) of three experiments.