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. 2011 Sep 27;2:484. doi: 10.1038/ncomms1495

Figure 6. eIF2α phosphorylation is influenced by the TOR complex signal.

Figure 6

L3–L4 males carrying the lin-32p∷eIF2α∷FLAG transgene (ncEx9002) were lysed, and FLAG-tagged eIF2α proteins were immunoprecipitated and subjected to immunoblot analysis with antibodies against total eIF2α or phospho-eIF2α. Black bars represent P-eIF2α in IP; grey bars, in lysate. Shown in the graphs are the means of normalized P-eIF2α±s.e.m. of three independent experiments. One asterisk indicates P<0.05; three asterisks, P<0.001. Paired t-test was carried out to calculate the P-values.