. 2011 Aug 26;438(Pt 3):427–432. doi: 10.1042/BJ20110953

Table 2. Ca²⁺-dependent [³H]PIP₂ hydrolysis activity and K_m of purified GST–PLC-fusion proteins.

Specific enzyme activity values obtained for the various GST–PLC-fusion proteins showing the EC₅₀ value of Ca²⁺-dependent enzyme activity and the Michaelis–Menten constant, K_m, for PIP₂ determined by non-linear regression analysis (GraphPad, Prism 5) for the GST–fusion proteins PLCζ, PLCζ^ΔXYl, PLCδ1, PLCδ1^ΔXYl and PLCζ^XYlδ1 (see Figure 3C).

GST–PLC protein	Ca²⁺-dependence EC₅₀ (nM)	Michaelis–Menten K_m (μM)
PLCζ	91	110
PLCζ^ΔXYl	84	3936
PLCδ1	6289	93
PLCδ1^ΔXYl	6973	63
PLCζ^XYlδ1	76	1909

Table 2. Ca2+-dependent [3H]PIP2 hydrolysis activity and Km of purified GST–PLC-fusion proteins.

Table 2. Ca²⁺-dependent [³H]PIP₂ hydrolysis activity and K_m of purified GST–PLC-fusion proteins.