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. Author manuscript; available in PMC: 2012 Oct 15.
Published in final edited form as: J Immunol. 2011 Sep 19;187(8):4347–4359. doi: 10.4049/jimmunol.1003404

Figure 6. ADO potentiates COX2 mRNA expression and PGE2 production induced by LPS stimulation.

Figure 6

COX2 mRNA quantification (A) and PGE2 production (B) were quantified in BMDCs pre-incubated with ADO or medium with or without LPS-stimulation for 24h. The indicated concentration of adenosinesynthetic analogous (2-phenylaminoadenosine) wasadded into BMDC culture 3h before LPS stimuli. PGE2 (C), TNF-α (D), and IL-10 (E) levels were detected 24 h later. PGE2 was measured by RIA and cytokines by ELISA assay. The results are expressed as the mean±SEM obtained from one of three independent experiments made in triplicate (N =3 per group). #, P<0.05 compared with medium; *, P<0.05 compared with the control (LPS).