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. Author manuscript; available in PMC: 2012 Nov 15.
Published in final edited form as: Anal Biochem. 2011 Jul 28;418(2):184–196. doi: 10.1016/j.ab.2011.07.024

Figure 3.

Figure 3

The dependences of fluorescence intensity after tagging of FSAY(3-NO2)LER with APPD on the concentration of K3Fe(CN)6 (A) or 3-NT peptide (B) and fluorescence spectra at different concentrations of 3-NT (C). A - FSAY(3-NO2)LER (5 µM) was reduced to FSAY(3-NH2)LER and incubated with 10 mM APPD and different concentrations of K3Fe(CN)6 in 0.1 M sodium phosphate buffer (pH 9.0) for 1 h. B and C - Fluorescence intensities and spectra, respectively, at different concentrations of the 3-NT peptide tagged with 10 mM APPD and 100 µM K3Fe(CN)6 as described above. Fluorescence was measured in a 0.5-mL (1-cm) cuvette using excitation and emission wavelengths of 360 and 510 nm, respectively.