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. Author manuscript; available in PMC: 2012 Nov 15.
Published in final edited form as: Anal Biochem. 2011 Jul 28;418(2):184–196. doi: 10.1016/j.ab.2011.07.024

Figure 4.

Figure 4

Analysis by MALDI-TOF MS (A) and reverse-phase HPLC detected by fluorescence (B) of APPD-tagged FSAY(3-NO2)LER. After reduction of 3-NT to 3-AT the peptide (10 µM) was incubated for 1 h with 10 mM APPD and 20 µM (curve 1) or 100 µM (curve 2) of K3Fe(CN)6 in 0.1 M sodium phosphate buffer (pH 9.0) at room temperature. Chromatograms were detected using excitation and fluorescence wavelengths of 360 and 510 nm, respectively.