Table 1.
E. coli strains used in this studya
| Strain | Relevant genotype | Reference or source |
|---|---|---|
| BW25113 | lacIq rrnB T14 ΔlacZWJ16 hsdR514 ΔaraBAD AH33 ΔrhaBAD LD78 | Datsenko and Wanner, 2000 |
| CAG12025 | zad-220::Tn10 panD | Singer et al., 1989 |
| JD20450b | dgt::mini-Tn10kan | Nat. Inst. Genet., Japanc |
| NR16947 | ara, thi, Δprolac, trpE9777, F'CC106 | Gawel et al., 2008 |
| NR17063 (HR44 but optA1) | thi-1 argH1 his-1 leu-6 lys-25 thr-1 ara-13 gal-6 lacY1 malA1 mtl xylA1 strR tonA optA1 | J. W. Drake |
| NR17624 | optA1 zad-220::Tn10 tonA panD | NR17063 × P1/CAG12025 |
| NR17642d | BW25113, but ΔthyA::kan | This work |
| NR17647 | optA1 zad-220::Tn10 panD tonA | NR16947 × P1/NR17624 |
| NR17648 | zad-220::Tn10 panD tonA | NR16947 × P1/NR17624 |
| NR17649 | optA1 zad-220::Tn10 panD tonA ΔthyA::kan | NR17647 × P1/NR17642 |
| NR17650 | zad-220::Tn10 panD tonA ΔthyA::kan | NR17648 × P1/NR17642 |
| NR17673 | NR17649, but kans | NR17649 × pCP20 |
| NR17674 | NR17650, but kans | NR17650 × pCP20 |
| NR17685e | BW25113, but ΔdeoD::kan | This work |
| NR17688 | optA1 zad-220::Tn10 panD tonA ΔthyA ΔdeoD::kan | NR17673 × P1/NR17685 |
| NR17689 | zad-220::Tn10 panD tonA ΔthyA ΔdeoD::kan | NR17674 × P1/ NR17685 |
| NR17698 | NR17688, but kans | NR17688 × pCP20 |
| NR17699 | NR17689, but kans | NR17689 × pCP20 |
| NR17726 | dgt::mini-Tn10kan zad-220::Tn10 panD tonA ΔthyA | NR17674 × P1/JD20450 |
| NR17738 | dgt::mini-Tn10kan zad-220::Tn10 panD tonA ΔthyA ΔdeoD | NR17699 × P1/JD20450 |
| NR17755f | BW25113, but ΔyjjG::cam | This work |
| NR17756 | optA1 zad-220::Tn10 panD tonA ΔthyA ΔyjjG::cam | NR17673 × P1/NR17755 |
| NR17757 | zad-220::Tn10 panD tonA ΔthyA ΔyjjG::cam | NR17674 × P1/NR17755 |
| NR17758 | dgt::mini-Tn10kan zad-220::Tn10 panD tonA ΔthyA ΔyjjG::cam | NR17726 × P1/NR17755 |
a
All strains are E. coli K-12 (λ−)
b
mini-Tn10kan insertion located at 228 nucleotides from start of dgt gene (insertion site 179,465)
c
National BioResource Project (NIG, Japan) (http://www.shigen.nig.ac.jp/ecoli/strain/top/top.jsp)
d
The mutant lacks 87% of thyA gene; kan cassette replaces nucleotides 2,962,433–2,963,127.
e
The mutant lacks 86% of deoD; kan cassette replaces nucleotides 4,618,906–4,619,625.
f
The mutant lacks 92% of yjjG; cam cassette replaces nucleotides 4,606,629–4,607,284.