Table 2.
Primers used for chromosomal gene deletions.
| Target gene | Pairs of primers (5'-3') | Templateb |
|---|---|---|
| thyA |
ATGAAACAGTATTTAGAACTGATGCAAAAAGTGCTCGACGAAGGCACACAGTGTAGGCTGGAGCTGCTTC TTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAAATTCCGGGGATCCGTCGACC |
pKD13 |
| deoD |
ATGGCTACCCCACACATTAATGCAGAAATGGGCGATTTCGCTGACGTAGTGTGTAGGCTGGAGCTGCTTC TTACTCTTTATCGCCCAGCAGAACGGATTCCAGTGCGATTTTGATCATGTATTCCGGGGATCCGTCGACC |
pKD13 |
| yjjG a |
TCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAGTGTAGGCTGGAGCTGCTTC CCAGTTCGTGCAACGAAGAAACGGTCCAGGTGGGCGCGATCATATGAATATCCTCCTTAG |
pKD3 |
a
Primers were designed according to Weiss, 2007a.