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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Mol Microbiol. 2011 Jul 18;81(3):831–849. doi: 10.1111/j.1365-2958.2011.07731.x

Figure 9. Expression of C-terminal truncations of Ssd1-GFP is not lethal in cbk1Δ cells.

Figure 9

Physiologically expressed Ssd11–570, Ssd11–670, Ssd11–1014 and Ssd11–1170 were monitored in cbk1Δ cells containing the nuclear marker NLS-RFP (pKW1219). None of the truncated Ssd1 proteins is toxic, in contrast to full length Ssd1 (not shown). Truncated Ssd1 proteins localize diffusely to the nucleus and to a few prominent foci, many of which localize in or near the nucleus. Up to 50% of the cytoplasmic puncta co-localize with P-body protein Edc3 (see Supplemental Fig. S3). See Table I for the strains used in these experiments. The cells were monitored by spinning disk fluorescence microscopy and each image represents a single optical section. Scale bar = 8 um.