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. Author manuscript; available in PMC: 2011 Oct 17.
Published in final edited form as: J Immunol. 2011 Mar 14;186(8):4551–4555. doi: 10.4049/jimmunol.1003573

Figure 4. CD4 T cell tissue antigen tolerance and de novo Foxp3 expression are undermined by inflammatory stimuli during priming.

Figure 4

Adoptive transfer of DR donor cells into RO/RAG° hosts as described in Figure 1. As indicated, chimeric mice were given 100µg of ovalbumin peptide with IFA s.c. at day 1 post-transfer (n=4), or 100µg of polyIC at days 1 and 3 (n=5) or days 80 and 82 post-transfer (n=7). (A) Diabetes incidence evaluated by blood-glucose monitoring as described in Figure 1, compiled data from two independent experiments. For comparison, dashed line depicts data from Figure 1 showing diabetes incidence of untreated chimeras. (B) SPL and PLN donor DR CD4 T cells were analyzed for Foxp3 expression at day 18 post-transfer, showing cells from untreated controls (ctrl) vs. cells from diabetic chimeras that had been treated with polyIC (polyIC) at days 1 and 3 post transfer, representative FACS plots shown, n=3 for each. (C) Frequency of Foxp3 expression among control and polyIC treated donor DR cells, compiled data from two independent experiments.