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. 2011 Apr 13;435(Pt 3):733–742. doi: 10.1042/BJ20102131

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© 2011 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Size-exclusion chromatography of (A) rHtrA_SA (B) rHhoA_SA and (C) rHhoB_SA. Black lines show elution profiles obtained in the absence of substrate, and grey lines show profiles after pre-incubation with β-casein in a 3:1 molar ratio of protease/substrate (A and B) or a 1:3 ratio (C). Lower panels show aliquots of the size-exclusion chromatography fractions separated by SDS/PAGE and visualized by Coomassie-Blue staining. The exclusion volume (V₀) of the columns and the elution volumes of selected marker proteins are indicated with black arrowheads. V_e, elution volume.