Figure 2. Ptf1a^cre/+;LSL-Kras^G12D/+;Tgfbr2^flox/flox PDAC cells produce and secrete Cxc chemokines and other soluble factors into the tumor microenvironment.

(A) A representative mouse cytokine array using CM from a Ptf1a^cre/+;LSL-Kras^G12D/+;Tgfbr2^flox/flox PDAC cell line (top) and a Ptf1a^cre/+;LSL-Kras^G12D/+cell line (bottom). 1, Cxcl1; 2, Cxcl2; 5, Cxcl5; 16, Cxcl16; G, Gcsf; V, Vcam-1. (B) Quantification of the mouse cytokine array data. The data from Kras mPanIN cells were set as 1, and relative quantity is shown. Kras+Tgfbr2^KO PDAC cells secreted higher levels of Cxc chemokines and other soluble proteins compared with Kras mPanIN cells. Kras indicates Ptf1a^cre/+;LSL-KrasG12D/+ mPanIN cell lines; Kras+Tgfbr2^KO indicates Ptf1a^cre/+;LSL-Kras^G12D/+;Tgfbr2^flox/flox PDAC cell lines. *P < 0.05; **P < 0.01; ***P < 0.001.