Figure 3. Transcriptional regulation of Cxc chemokines in the PDAC and mPanIN cells.

(A) Cxc chemokines were upregulated at the mRNA level in the Kras+Tgfbr2^KO PDAC cells compared with Kras mPanIN cells in QRT-PCR analysis. The data of Kras mPanIN cells was assigned as 1, and relative quantity is shown. (B) Cxcl1 and -5 mRNA expression was downregulated by TGF-β signaling in the Kras mPanIN cell lines, but not in the Kras+Tgfbr2^KO PDAC cells. Cells were incubated with or without 5 ng/ml TGF-β1 for 6 hours, and QRT-PCR was performed. The data without TGF-β1 was assigned as 1, and relative quantity is shown. (C) The upregulation of Cxcl1 and -5 in Kras+Tgfbr2^KO PDAC cells was NF-κB signal dependent. Kras+Tgfbr2^KO PDAC cells (K375 and K399) were incubated with NF-κB inhibitor SC-514 (0–100 μM) for 12 hours, and QRT-PCR was performed. Data without an inhibitor were set as 1, and relative quantity is shown. *P < 0.05; **P < 0.01; ***P < 0.001.