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. 2011 Sep 19;121(10):3846–3859. doi: 10.1172/JCI44952

Figure 8. Antigen-restricted killing of tumor targets depends on CD8+ T cell trafficking across tumor vessels.

Figure 8

(A) Growth curves of B16-OVA tumors in mice treated with STT and adoptive cell transfer (ACT) of OT-I effector cells. Treatment times are denoted by arrows; numbers above arrows indicate tumor volume at the time of treatment. n = 5 mice per condition. *P < 0.04 versus NT plus ACT. (B) Representative photomicrographs of B16-OVA tumors 12 hours after adoptive transfer of TRITC-labeled OT-I cells (red). Apoptotic cells were detected by TUNEL assay (green), and nucleated cells were stained with DAPI (blue). Scale bar: 100 μm. Quantification of intratumoral TRITC+ OT-I cells and TUNEL+ apoptotic cells at 1 or 12 hours after adoptive transfer is also shown. (C) Neutralizing antibody specific for IL-6 or E/P-selectin was administered intravenously 30 minutes before STT treatment in B16-OVA tumor–bearing WT mice. Tumors were also implanted in Icam1–/– mice. (B and C) Data are from individual mice and are representative of at least 3 independent experiments. *P < 0.002 versus NT.