Figure 6. LZT is associated with increased TNF/p75-induced apoptosis in CHS effector CD8⁺ T cells.

(A–D) Apoptosis in lymph node cells obtained 24 hours after challenge from tolerized and sensitized Tnf^–/– and corresponding WT mice (A and B) and from p75^–/– and corresponding WT mice (C and D). CD8⁺ T cell apoptosis was detected by flow cytometry (annexin V staining, pooled cells obtained from 5–6 animals). 1 of 4 experiments with similar results (A and C) and pooled data of 4 experiments (B and D) are shown. (E and F) Percentages of apoptotic Thy1.2⁺ CD8⁺ T cells as assessed by flow cytometry (annexin V⁺/CD8⁺) in lymph node cells obtained after challenge with TNCB from TNCB-tolerized or mock-tolerized Thy1.1⁺ mice that were then reconstituted with T cells isolated from TNCB-sensitized Thy1.2⁺ mice (E) or in lymph node cells obtained after challenge with DNFB from TNCB-tolerized or mock-tolerized Thy1.1⁺ mice that had been reconstituted with T cells isolated from DNFB-sensitized Thy1.2⁺ mice (F). For analyses, gate was set on Thy1.2⁺ T cells (derived from the sensitized donor mice). 1 of 3 independent experiments with similar results is shown. 5 per group per experiment were used. Data are shown as mean ± SD. *P < 0.05; **P < 0.01.