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. 2011 Sep 12;121(10):4127–4137. doi: 10.1172/JCI46458

Figure 2. Arhgap24 transcript and Arhgap24 protein are specifically expressed in podocytes.

Figure 2

(A) MBEI values for microarray data for in vitro–cultured podocytes (33°C, 37°C), ex vivo–isolated podocytes (E13.5, E15.5, and adult), and laser-capture-microdissected glomeruli (E12.5 renal vesicle, E15.5 S-shaped body, and E15.5 renal corpuscle) show an increase in the Arhgap24 transcript with differentiation in vitro and in vivo. (B) By quantitative RT-PCR, differentiated podocytes have higher Arhgap24 mRNA levels compared with those of undifferentiated podocytes, after normalization to 18S rRNA. (C) Immunoblotting shows that differentiated podocytes have higher levels of Arhgap24 protein than undifferentiated podocytes. (D) Confocal imaging of cultured podocytes also shows an increase in levels of Arhgap24 in differentiated podocytes, concentrated in punctate structures at the base of the cell. (E) Arhgap24 colocalizes with the focal adhesion marker vinculin at the tips of actin stress fibers. Original magnification, ×600 (D and E).