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. 2011 Sep 12;121(10):4127–4137. doi: 10.1172/JCI46458

Figure 5. Arhgap24 knockdown in differentiated podocytes increases active Rac1 and Cdc42 levels and accelerates epithelial monolayer wound closure.

Figure 5

(A) Pull down of active (GTP-bound) Rac1, Cdc42, and RhoA shows that the Arhgap24 knockdown cell lines (lines 451 and 756) have increased levels of active Rac1 and Cdc42 compared with those of the control (Fluc). However, active RhoA levels are similar across all 3 cell lines. Results are representative of 3 independent experiments. (B) Arhgap24 knockdown cells migrate and close a scratch made in a confluent monolayer faster than the control knockdown cell line. Cell nuclei were stained with Hoechst dye. Original magnification ×100.