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. 2011 Sep 12;121(10):4127–4137. doi: 10.1172/JCI46458

Figure 7. Arhgap24 Q158R has defective Rac1-GAP activity and dimerizes with the wild-type protein.

Figure 7

(A) Wild-type FLAG-tagged Arhgap24 (first lane) transfected into HEK293 cells reduces active Rac1 levels compared with Q156R Arhgap24 (last lane). Titration of increasing proportions of Q156R Arhgap24 produces increased levels of active Rac1 (middle lanes). Total Rac1 and FLAG-Arhgap24 protein levels are similar across all lanes. Results are representative of 3 different experiments. (B) FLAG- or GFP-tagged wild-type (W) or Q158R Arhgap24 (Q) constructs were cotransfected into HEK293 cells. Cell lysates were immunoprecipitated with anti-FLAG antibody and then immunoblotted for GFP-tagged Arhgap24 to assess for protein dimerization. Whole cell lysates (WCLs) were immunoblotted for GFP and FLAG to ensure protein expression.