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. 2011 Sep 1;121(10):3954–3964. doi: 10.1172/JCI57362

Figure 6. Induction but not maintenance of the antibody response to influenza is dependent on T cell help.

Figure 6

(A) WT and TACI–/– mice (n = 7–8 mice/group) were infected with influenza virus PR8. Starting on day 3 p.i., WT and TACI–/– mice received 5 i.p. injections of MR1 or hamster Ig control Ab (150 μg/mouse) in 3-day intervals. Blood samples were collected prior to (day 0) and days 8, 14, 21, 30, and 45 after infection and PR8-specific IgG Ab titers were determined by ELISA. (B–D) BLIMP-1–YFP mice were infected with influenza virus PR8 and treated 3 times in 3-day intervals starting on day 38 p.i. with MR1 or hamster Ig Ab (150 μg/mouse). At day 46 p.i., spleen, medLN, lungs, and BM were harvested. Diagram in panel B applies to panels B–E. (B) YFP+ ASCs were enumerated by flow cytometry and (C) PR8-specific ASCs determined by ELISPOT. (D) PR8-specific IgG Ab titers in serum and (E) IgG and IgA in BAL were measured by ELISA. All data with mean ± SEM combine the results of 2 independent experiments. *P < 0.05, ***P < 0.001.