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. 2011 Sep 12;121(10):4095–4105. doi: 10.1172/JCI58818

Figure 4. TRAF6 is upstream of NF-κB in human lung cancer cells.

Figure 4

(A) NF-κB activity was measured by κB-site luciferase assays in human lung cancer cells with (HCC95, H526, H2171, HTB58) and without (H2170, H2347, H520, HTB117) amplification of the TRAF6 locus (11p13). (B) Nuclear NF-κB (p65) was IB in nuclear extracts from 7 human lung cancer cell lines with (H2170, H2347, H520) or without (H2170, H2347, H520, HTB117) amplification at the TRAF6 locus (11p13). (C) H460 (diploid at 11p13) and HCC95 (amplification of 11p13) were evaluated for κB-site luciferase activity and for TRAF6 protein expression. (D) NF-κB activity was measured by κB-site luciferase assays after TRAF6 inhibition. *P < 0.05.

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