Table 1. The compound database.
A dataset of 23 collagen IV derived compounds tested for endothelial cell proliferation (at 100 mg/ml), migration (at 50 mg/ml), and adhesion (at 100 mg/ml) inhibition. The table gives the mean % inhibition for each assay and the standard error of the mean (SEM). The screening of all compounds was done with n=2 and normalized to a vehicle control. We use the single letter X to represent L-α-amino-n-butyric acid (Abu). Peptides 27 and 35 were held out as an external validation set.
| Ref. | Compound | Structure | Proliferation | Migration | Adhesion |
|---|---|---|---|---|---|
| 0 | SP2000 | LRRFSTMPFMFCNINNVCNF | 67.55 ± 2.58 | 91.02 ± 0.63 | 96.25 ± 0.34 |
| 2 | SP2002 | LRRFSTMPFMFGNINNVGNF | 25.71 ± 0.78 | 87.90 ± 0.70 | 97.80 ± 0.15 |
| 4 | SP2004 | LRRFSTMPFMF********* | 2.97 ± 2.62 | −14.20 ± 7.85 | −2.60 ± 0.32 |
| 6 | SP2006 | LRRFSTMPFMFXNINV**** | 59.70 ± 4.10 | 26.55 ± 3.15 | 95.62 ± 1.22 |
| 7 | SP2007 | LRRFSTMPFMFX******** | 4.85 ± 1.62 | −24.38 ± 5.87 | 11.04 ± 1.81 |
| 8 | SP2008 | LRRFSTMP************ | 7.02 ± 0.69 | −20.09 ± 4.67 | −0.14 ± 0.00 |
| 9 | SP2009 | ************NINNVXNF | 24.25 ± 12.25 | 31.65 ± 0.85 | 70.78 ± 0.05 |
| 10 | SP2010 | ********FMFXNINNVXNF | 28.30 ± 3.39 | −16.60 ± 9.83 | 66.18 ± 1.83 |
| 11 | SP2011 | ****STMPFMFXNINNVXNF | 13.83 ± 7.64 | −24.46 ± 6.89 | 6.82 ± 2.87 |
| 12 | SP2012 | LRRFSTMPFMFXNINNVXNF | 69.72 ± 2.25 | 96.97 ± 0.19 | 98.46 ± 0.32 |
| 13 | SP2013 | LNRFSTMPF*********** | 6.35 ± 4.17 | −16.05 ± 0.69 | 0.32 ± 0.86 |
| 14 | SP2014 | LRRFSTNLPFNLF******* | 2.95 ± 3.01 | −22.77 ± 1.28 | −24.59 ± 15.37 |
| 15 | SP2015 | LRRFSTMPAMFXNINNVXNF | 65.40 ± 1.20 | 99.75 ± 0.25 | 95.60 ± 0.31 |
| 16 | SP2016 | LRRFSTMPFAFXNINNVXNF | 63.60 ± 0.35 | 99.60 ± 0.40 | 98.33 ± 1.14 |
| 17 | SP2017 | LRRFSTMPFMAXNINNVXNF | 65.10 ± 0.57 | 99.60 ± 0.40 | 99.27 ± 0.21 |
| 20 | SP2020 | **********FXNINNVXN* | 20.55 ± 0.46 | 40.35 ± 3.45 | 77.51 ± 2.36 |
| 21 | SP2021 | **********FXNIN***** | 8.17 ± 2.27 | −23.68 ± 0.59 | 3.45 ± 2.25 |
| 22 | SP2022 | LRRFSTMPFMFSNINNVSNF | 50.47 ± 0.66 | 92.56 ± 0.97 | 97.95 ± 1.27 |
| 23 | SP2023 | LRRFSTMPFMFANINNVANF | 48.61 ± 0.53 | 99.59 ± 0.13 | 98.19 ± 0.03 |
| 24 | SP2024 | LRRFSTMPFMFININNVINF | 73.20 ± 0.78 | 92.92 ± 1.78 | 98.58 ± 0.12 |
| 25 | SP2025 | LRRFSTMPFMFTNINNVTNF | 59.10 ± 0.85 | 96.08 ± 0.15 | 98.69 ± 0.08 |
| 27 | SP2027 | LRRFSTMPFMFVNINNVVNF | 62.20 ± 0.64 | 98.78 ± 0.14 | 95.12 ± 0.62 |
| 35 | SP2035 | LRRFSTMPFAFININNVINF | 46.58 ± 4.23 | 69.33 ± 0.14 | 94.80 ± 0.93 |