FIGURE 2.

RXLR effectors can adopt different oligomeric states in vitro and in vivo. Fits of the analytical ultracentrifugation data (including residuals) for (a) AVR3a11 (0.3 mg/ml (circles) and 0.45 mg/ml (triangles)) (b) PexRD2 (3 mg/ml (triangles) and 6 mg/ml (circles)) to the models described confirmed that monomeric and dimeric species are the prevalent forms of these proteins in solution. c, co-immunoprecipitation (co-IP) shows that PexRD2 self-associates in planta; no oligomerization is apparent for AVR3a11, AVR3a^KI, or AVR3a^EM. RuBisCO is included as a loading control (Coomassie-stained SDS-polyacrylamide gel).