TABLE 1.
Comparison of spectral properties for various PE subunits produced with bilin lyases
| Plasmids used for Apoproteins | Plasmids used for bilin lyases | λmax (Q Vis/UV)a | Fluorescence emission, λmax | Фf | Fluorescence emission |
|---|---|---|---|---|---|
| nm | % | ||||
| pCpeA | pCpeYZb | 566/410 (5.4) | 574 | 0.72 | 100 |
| pCpeA(C82S) | pCpeYZb | NAc | NA | ND | NDd |
| pCpeA(C139S) | pCpeYZb | 566/410 (5.6) | 574 | ND | ND |
| pCpeA(C82S/C139S) | pCpeYZb | NA | NA | ND | ND |
| pCpeA | pCpeZb | NA | NA | ND | 0 |
| pCpeA | pCpeYb | 566/410 (15.6) | 574 | ND | 60 |
| pCpeA(C82S) | pCpeYb | NA | NA | ND | ND |
| pCpeA(C139S) | pCpeYb | 566/410 (14.8) | 574 | ND | ND |
| pCpeA(C82S/C139S) | pCpeYb | NA | NA | ND | ND |
| pCpeA | pCpeSb | 561/410 (0.315) | 574 | 0.89 | 0.8 |
| pCpeA(C82S) | pCpeSb | 550/398 (0.6) | 562 | ND | ND |
| pCpeA(C139S) | pCpeSb | 562/410 (0.6) | 574 | ND | ND |
| pCpeA(C82S/C139S) | pCpeSb | NA | NA | ND | ND |
| pCpeB | pCpeSb | 560/412 (5.2) | 571 | ND | ND |
| pCpeB(C80S) | pCpeSb | NA | NA | ND | ND |
| pCpeB(C165S) | pCpeSb | 560/412 (5.4) | 571 | ND | ND |
| pCpeB(C48S/C59S) | pCpeSb | 560/398 (5.3) | 571 | ND | ND |
| Native PE (αβ)6e | 563/374 (9.5) | 573 | ND | ND | |
a Q Vis/UV denotes the absorbance ratio of the visible and near-UV bands.
b Coexpressed with pPebS.
c Not applicable because there was no fluorescent product produced.
d ND, not determined.
e Holo-PE purified from F. diplosiphon.