FIGURE 1.

Mass spectrometric analysis of H. roretzi mt tRNAs. A, base peak chromatograms (BPC) of RNase T₁-digested fragments are shown for tRNA^Gly(AGR) (top), tRNA^Met(AUR) (middle), and tRNA^Trp(UGR) (bottom). Mass chromatograms for the anticodon-containing fragments of each tRNA are also indicated. Sequences and molecular masses of the RNA fragments numbered on base peak chromatograms are listed in supplemental Table 1. The anticodons are underlined. B, collision-induced dissociation spectrum of the anticodon-containing fragment derived from tRNA^Gly(AGR). The triply charged negative ion (m/z 1200.8) was employed as a precursor ion for collision-induced dissociation. Product ions of the c- and y-series (21) are indicated on the spectrum and assigned to the sequence.