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SDS-PAGE analysis of WT and N149C mutant BhAcot. Left panel, oxidation of the disulfide linkage over time. Lane 1, time 0, prior to removal of reducing agent; lanes 2-4, 1, 3, and 5 days after removal of reducing agent, respectively; lane 5, the addition of reducing agent to the oxidized protein (day 5) shows reduction to monomeric mutant BhAcot. Right panel, analysis of fractions from size exclusion chromatography including boiling and non-boiling heat treatments.
