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Summary of kinetic values for acyl-CoA thioesterase activity of BhAcot against a range of fatty-acyl CoA substrates
ND, not determined due to low catalytic properties.
| Acyl-CoA substrate | Vmax | Km |
|---|---|---|
| μmol/min/mg | μmol | |
| Butyryl-CoA | 50 ± 2.5 | 64 ± 9 |
| Propionyl-CoA | 33 ± 9 | 213 ± 100 |
| Acetyl-CoA | 14 ± 2.5 | 163 ± 56 |
| Malonyl-CoA | <10 | ND |
| Succinyl-CoA | <10 | ND |
| Octanoyl-CoA | <10 | ND |
| Lauryl-CoA | <10 | ND |
| Palmitoyl-CoA | <10 | ND |
| Arachidonoyl-CoA | <10 | ND |
| Arachidoyl-CoA | <10 | ND |
