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. 2011 Aug 11;286(41):35367–35379. doi: 10.1074/jbc.M110.214148

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Glycolipid synthesis in recombinant E. coli. A, TLC of lipid extracts from E. coli cells expressing mg517, mg060, and mg025 genes, respectively. BL21 are control cells transformed with the pET38b(+) with no insert. B, TLC-autoradiography of in vivo glycolipids from M. genitalium grown in [¹⁴C]glucose medium (lane 1 labeled Myc) and in vitro products from GT assays with spheroblasts of E. coli cell expressing mg517, mg060, and mg025 genes incubated with UDP-[¹⁴C₆]Glc, DOG, and SDS. GL1, GL2, and GL3, glycolipids; TG, triglycerides.