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Time course monitoring of GT activity in recombinant E. coli spheroblasts expressing mg517. A, in vitro GT assay: TLC-autoradiography of the reaction mixture containing E. coli-pET38b(+)-mg517, UDP-[14C6]Glc, and DOG in SDS at increasing reaction times. B, in vivo glycolipid biosynthesis; TLC of a lipid extract of E. coli cell expressing mg517 stained for glycolipids with N,N-diphenylamine.
