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. 2011 Aug 11;286(41):35367–35379. doi: 10.1074/jbc.M110.214148

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — GT MG517 kinetics with UDP-Glc and UDP-Gal donors and DOG and MGlcDOG acceptors at pH 8.0, 35 °C. A and B, monoglycosyltransferase activity with DOG acceptor. A, varying UDP-Gal (or UDP-Glc; inset) at constant 1.25 mm (3.7 mol%) DOG. B, dependence on DOG concentration at constant 1 mm UDP-Gal. C and D, diglycosyltransferase activity with MGlcDOG acceptor. C, varying UDP-Gal (or UDP-Glc; inset) at constant 1.25 mm (3.7 mol %) MGlcDOG. D, dependence on MGlcDOG concentration at constant 1 mm UDP-Gal. Fitted parameters are given in Table 3.