TABLE 1.
Characterized glycosyldiacylglycerol synthases grouped by CAZyme GT families
Phylogenetic trees for glycosyldiacylglycerol synthases (UNIPROT sequences, both characterized and uncharacterized) are provided in supplemental Fig. S4).
| Family | Activity/EC | Organism | Enzyme | UNIPROT | Product | Reference |
|---|---|---|---|---|---|---|
| GT28a | MGalDAG synthase/EC 2.41.1.46 | Arabidopsis thaliana | Mgd1 | O81770 | Galβ3DAG | 10, 15 |
| A. thaliana | Mgd2 | O82730 | Galβ3DAG | 10, 12 | ||
| A. thaliana | Mgd3 | Q9SI93 | Galβ3DAG | 10, 12 | ||
| Cucumis sativus | MGD1 | P93115 | Galβ3DAG | 9 | ||
| Spinacia aleracea | MGD1 | Q9SM44 | Galβ3DAG | 13 | ||
| MGalD synthase/EC 2.41.1.46 | Chloroflexus aurantiacus | MGD | A9WFF3 | Galβ3DAG | 14 | |
| DGlcDAG/EC 2.4.1.208 | C. aurantiacus | DGD | A9WFF4 | Glcβ6Galβ3DAG | 14 | |
| Processive GlcDAG synthase | Bacillus subtilis | YpfP | P54166 | Glcβ3DAG, Glcβ6Glcβ3DAG | 20 | |
| S. aureus str. RN4220 | YpfP | Q5HH69 | Glcβ3DAG, Glcβ6Glcβ3DAG | 21 | ||
| GT21 | Procesive Glc/Gal synthase | Agrobacterium tumefaciens | AtGcs | Q7CYH2 | Glcβ3DAG, Galβ3DAG, Glcβ6Glcβ3DAG, Glcβ6Galβ3DAG, Galβ6Galβ3DAG | 22 |
| Mesorhizobium loti | MlGcs | Q98BB3 | Same as above | 22 | ||
| GT4b | DGalDAG synthase/EC 2.4.1.241 | A. thaliana | DGDG1 | Q9S7D1 | Galα6Galβ3DAG | 17 |
| MGlcDAG synthase/EC 2.4.1.157 | A. laidlawii | AlMgs | Q93P60 | Glcα3DAG | 33 | |
| Streptococcus pneumoniae | SpMgs | O06453 | Glcα3DAG | 33 | ||
| Deinococcus radiodurans | DrMgs | Q9RV05 | Glcα3DAG | 14 | ||
| Thermotoga maritima | TrMgs | Q9WZK3 | Glcα3DAG | 14 | ||
| DGlcDAG synthase/EC 2.4.1.208 | A. laidlawii | AlDgs | Q8KQL6 | Glcα2Glcα3DAG | 36 | |
| S. pneumoniae | CpoA | O06452 | Galα2Glcα3DAG | 52 | ||
| GT2 | MGlcDAG synthase/EC 2.4.1.157 | Synechocystis sp. | MGlcDG | P74165 | Glcβ3DAG | 18 |
| Nostoc sp. PCC 7120 | MGlcDG | Q8YMK0 | Glcβ3DAG | 18 | ||
| Procesive Glc/Gal synthase | M. pneumoniae | MPN483 | P75302 | Galβ3DAG, Glcβ6Galβ3DAG | 38 | |
| M. genitalium | MG517 | Q9ZB73 | Glcβ3DAG, Galβ3DAG, Glcβ6Glcβ3DAG | This work |
a Other annotated (UNIPROT), not characterized GT28 (plant): Glycine max (Q9FZL4), Nicotina tabacum (Q9FZL3), Oryza sativa subsp. Japonica (type 1, Q69QJ7 ; type 2, Q6UTZ2 ; type 3, Q0DWQ1), O. sativa subsp. Indica (type 2, A2YTP9), Vigna unguiculata (type A, Q07A03; type B, A4UNX3, fragment).
b Other annotated (UNIPROT), not characterized GT4 (plant): DGalDAG synthase: A. thaliana (type 2, Q8W1S1), Lotus japonicus (type 2, Q6DW73), L. japonicus (type 1, Q6DW74), G. max (type 1, Q6DW76), G. max (type 2, Q6DW75), N. tabacum (Q6DQ98), V. unguiculata (type 1, Q07A01), V. unguiculata (type 2, A4UNX4, fragment); Xerophyta humilis (Q84XL8). Other annotated (UNIPROT), not characterized GT4 (cyanobacteria): DGalDAG synthase Synechocystis sp. PCC 6803 (P73948).