TABLE 2.

Dyngo-4a delays the onset of BoNT/A-induced paralysis in the phrenic nerve-hemidiaphragm twitch preparation

Because each muscle contracts with a different strength, the data from the Dyngo-4a-treated and untreated BoNT/A muscles were normalized and expressed as a percentage. The data were then fitted to a four-parameter logistic curve as described under “Experimental Procedures.” Statistical analysis of the t½ and the Hill slope revealed they were significantly different (**, p < 0.01). This indicates that Dyngo-4a delays the onset of BoNT/A-induced paralysis and slows the rate of decline.

Treatment	BoNT/A	Dyngo-4a + BoNT/A
Minimum	15.7 ± 2.2	10.6 ± 6.9
Maxium	95.3 ± 1.9	102.9 ± 1.8
T½	247.4 ± 2.2	302.7 ± 10.0**
Hill slope	5.83 ± 0.43	3.73 ± 0.33**
R2	0.9994	0.9999
N	6	9