TABLE 1.
Absorption spectra of the Fe(III), Fe(II), Fe(II)-O2, and Fe(II)-CO complexes of wild-type and mutant AfGcHK
Corresponding spectra of other heme-based oxygen sensor enzymes and SWMb are shown as the reference. Proposed coordination structures are presented in parentheses. 6cLS, 6-coordinated low spin; 5cHS, 5-coordinated high spin; 6cHS, 6-coordinated high spin.
| Proteins | Fe(III) | Fe(II) | Fe(II)-O2 | Fe(II)-CO |
|---|---|---|---|---|
| AfGcHK | ||||
| WT | 411, 538 (6cLS) | 431, 559 (5cHS) | 413, 545, 580 (6cLS) | 420, 541, 565 (6cLS) |
| Y45F | 411, 535 (6cLS) | 430, 555 (5cHS) | 414, 546, 579 (6cLS) | 420, 541, 563 (6cLS) |
| Y45L | 410, 534 (6cLS) | 431, 564 (5cHS) | 415, 548, 578 (6cLS) | 421, 541, 565 (6cLS) |
| Y45W | 410, 534 (6cLS) | 431, 558 (5cHS) | 414, 547, 580 (6cLS) | 420, 543, 565 (6cLS) |
| H99A | No heme absorption | |||
| H183A | 411, 543 (6cLS) | 432, 561 (5cHS) | 413, 545, 580 (6cLS) | 421, 541, 565 (6cLS) |
| YddVa | 394, 506, 651 (5cHS) | 432, 560 (5cHS) | 413, 542, 578 (6cLS) | 420, 539, 566 (6cLS) |
| HemAT-Bsb | 402, 505, 640 (6cHS) | 431, 563 (5cHS) | 414, 543, 578 (6cLS) | 422, 543, 567 (6cLS) |
| SWMbc | 410, 505, 635 (6cHS) | 434, 556 (5cHS) | 418, 543, 581 (6cLS) | 423, 542, 579 (6cLS) |
| EcDOSd | 417, 530, 562 (6cLS) | 428, 532, 563 (6cLS) | 417, 542, 578 (6cLS) | 424, 542, 578 (6cLS) |
| BjFixLe | 395, 509, 645 (5cHS) | 437, 556 (5cHS) | 419, 545, 562 (6cLS) | 427, 548, 560 (6cLS) |